XB-IMG-154153
Xenbase Image ID: 154153
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Fig. 4. xSOUL is a novel secreted protein of
the SOUL/Heme-binding protein family. (A)
Culture medium of 293T cells transfected with
green fluorescent protein cDNA as control (
GFP) or Xenopus SOUL cDNA (xSOUL ). xSOUL
is secreted as a 23 kD protein. (B) Evolutionary
relationship of xSOUL and other members of
the SOUL/heme binding protein (HBP) family.
Proteins with the greatest sequence similarity
cluster together and branch lengths are proportional
to distance (TreeTop-Phylogenetic Tree
Prediction; http://www.genebee.msu.su/services/
phtree_reduced.html). Only the mature
proteins have been considered. Note that xSOUL
is most closely related to a derived family member
in the plant Arabidopsis thaliana (aSOUL).
The listed proteins have the following GenBank
accession numbers: xSOUL (GenBank accession
number DQ004682); aSOUL (NM101570);
human SOUL (AF117616); mouse SOUL
(AF117614); zebrafish SOUL (BC045936); human
HBP (AF117615); mouse HBP (AF117613);
chick SOUL (AF117612). (C) Comparison of
xSOUL with other SOUL/HBP sequences. Note
that only xSOUL and aSOUL have cleavable
signal peptides (triangle). The putative hemebinding
region as reported by Zylka and Reppert
(1999) is overlined. (D) Four-cell stage embryo
in animal view showing maternal expression of
xSOUL. (E) Anterior view of advanced neurula.
Note weak expression in the anterior brain
(arrowhead). (F) Tailbud stage embryo with
distinct expression in the forebrain, lens, somites
and gut. Image published in: Pera EM et al. (2005) Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press.
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