Xenbase Image ID: 154154

Fig. 5. Xystatin is a novel secreted cysteine proteinase inhibitor. (A) Supernatant of 293T cells non-transfected (control) or transfected with Xenopus Cystatin cDNA (xCys ). Note that xCys is secreted as an 18 kDa protein. (B) Phylogenetic tree indicating the relationship of xCys with other members of the cystatin superfamily. Only the mature proteins lacking the signal peptide have been aligned. GenBank accession numbers are as follows: Xystatin (DQ004681); salmon Cystatin (D86628); chick Cystatin (JO5077); murine Cystatin-C (NM009976); human Cystatin-C (BC013083). (C) Sequence alignment of Xcys and related members of the cystatin superfamily. Four conserved cysteine residues known to form two disulphide bonds are labeled with stars. The conserved active site sequence QxVxG and pro-trp (PW) sequences are underlined (Brown and Dziegielewska, 1997). Image published in: Pera EM et al. (2005) Copyright © 2005. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View

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