Xenbase Image ID: 154157

Fig. 2. Six1 binds Pa2G4. (A) HEK 293T cells were transfected with six1-Flag and pa2g4-HA plasmids. Pa2G4-HA was detected by Western blot (WB) when Six1-Flag was immunoprecipitated with an anti-Flag antibody (IP). (B) HEK 293T cells were transfected with equimolar amounts of six1-Flag and eya1-myc plasmids. In addition they were transfected with varying relative amounts of pa2g4-HA plasmids: equal (1.0), half (0.5), quarter (0.25), eighth (0.125) or none (0). Eya1-Myc was detected by Western blot (WB) when Six1-Flag was immunoprecipitated (IP). Eya1 bound to Six1 when Pa2G4 levels were low (0.125 or 0.25 M ratios), but did not bind when Pa2G4 levels were increased (0.5 or 1.0 M ratios). Left-most lane shows no transfection. Image published in: Neilson KM et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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