Xenbase Image ID: 154163

Fig. 8. Pa2G4 levels affect cell death and cell proliferation. (A) Knock-down of endogenous Pa2G4 by MO injection significantly reduces the number of apoptotic cells in the neural crest/PPE domain, as indicated by the TUNEL assay. Loss of Pa2G4 also significantly reduces the number of mitotic cells, as indicated by phosphorylated H3 (PH3) immunostaining. Embryos evaluated at stage 16. (B) Increasing levels of Pa2G4 by mRNA injection (100 pg, 200 pg, or 400 pg) has no significant effect on proliferation in the neural crest/PPE domain when embryos are evaluated at stage 18. Ctrl, control side; KD, knock-down side; inj, mRNA-injected side. *, p<0.05, paired t-test. Image published in: Neilson KM et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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