Xenbase Image ID: 154164

Supplemental Figure 1: In Xenopus, there are two homeologues of pa2g4, one on the short chromosome (pa2g4S) and one on the long chromosome (pa2g4L). The nucleotide sequence surrounding the translational start site (ATG) of each is presented. Above these sequences (in blue) are the sequences of the two anti-sense Morpholino oligonucleotides (MO) that were used to knock-down endogenous Pa2G4 expression. MO#1 binds to both the short and long pa2g4 mRNAs within the open reading frame. MO#2 will bind to pa2g4S, but pa2g4L contains 5 mismatches (in red). The sequence of the rescue mRNA used to reverse the effects of MO knock-down is shown (pa2g4 rescue mRNA). This mRNA is insensitive to both MOs (mismatches in red) due to removal of the endogenous 5′UTR, insertion of the pCS2+ polylinker upstream of the ATG and insertion of an HA-tag downstream of the ATG that separates the MO binding from the open reading frame; MOs that bind more than 30 bases from the translational start codon do not block translation (www.gene-tools.com/choosing_the_optimal_target#TranslationalBlocking). Image published in: Neilson KM et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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