XB-IMG-154165
Xenbase Image ID: 154165
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Supplemental Figure 2: Validation of efficacy and specificity of pa2g4 MOs. (A) Expression of an mRNA that contains the native pa2g4S 5′ UTR and a 3′HA tag (5′UTR 2g4) expresses at high levels when injected into oocytes (duplicate lanes 2 and 3). Its expression is prevented by co-injection with MO#1+ MO#2 (duplicate lanes 4 and 5). uninj = uninjected oocyte lysate (lane 1). Numbers to the left indicate molecular weights. (B) Expression of an mRNA that is missing the native 5′UTR (rescue 2g4) expresses at high levels when injected into oocytes (duplicate lanes 1 and 2). Its expression is unaffected by co-injection with MO#1+ MO#2 (duplicate lanes 3 and 4). (C) Neural crest expression of foxd3 (blue stain on control [ctrl] side) is undetected on the MO-injected side (MOs) in a high percentage of embryos (n=66). (D) Neural crest expression of foxd3 (blue stain) is restored on the MO injected side by co-expression of an MO-insensitive mRNA (rescue) in a high percentage of embryos (n=24). Image published in: Neilson KM et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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