Xenbase Image ID: 154185

Fig. 3. Inhibition of BMP signaling rescues the craniofacial defects observed in the tadpoles homozygous for the nogRMH3allele. (A–D) Untreated tadpoles at stage 45 (E–H) Tadpoles at stage 45 treated with 0.1% DMSO for 24 h at stage 28. (I–L) Stage 45 tadpoles treated with 10 μM LDN-193189 for 24 h at stage 28. Genotyping showed that the following representative embryos are wild-type (A–B,E–F,I–J), while (C–D,G–H,K–L) are homozygous nogRMH3 tadpoles. (A,C,E,G,I,K) Dorsal views. (B,D,F,H,J,L) lateral views. White arrowheads show protruding suprarostral plate in the mutants, red arrowheads show rescued structures in mutants following treatment. (M) Quantification of phenotypes observed following treatments indicated. Black bars show the number of tadpoles with a wildtype phenotype, open bars show the number with the mutant phenotype and gray bars indicate tadpoles with non-specific defects. ** indicates p<0.0001. Image published in: Young JJ et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page