Xenbase Image ID: 154225

Figure 1. Expression profiles of genes involved in Notch signaling in X. laevis intestine during natural and T3-induced metamorphosis. Quantitative real-time RT-PCR was performed using total RNAs prepared from the intestine of animals at indicated developmental stages (A, C, E, G, I, K, M) or stage-54 tadpoles after 10 nM T3 treatment (B, D, F, H, J, L, N). Levels of Hairy1 (A, B), Hairy2b (C, D), DLL1 (E, F), DLL3 (G, H), Jag1 (I, J), Jag2 (K, L), and Notch1 (M, N) mRNAs are shown relative to those of ribosomal protein L8 (rpL8) mRNA, with the values at stage 54 or 0-day treatment set to 1. Error bars represent the SEM (n = 7 for A-D; n = 3 for E-H, J, M, N; n = 8 for I, L; n = 5 for K). The values were analyzed by ANOVA followed by Scheffe's post hoc test whose results are shown only for the adjacent stages or days except for Notch1 (M). Asterisks indicate that the mRNA levels are significantly different. *, p < .05, **, p < .01; ns: not significant. Image published in: Hasebe T et al. (2017) © 2016 The Authors STEM CELLS published by Wiley Periodicals, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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