Xenbase Image ID: 154226

Figure 2. Localization of Hairy1 and Hairy2b mRNAs in the X. laevis intestine during natural and T3-induced metamorphosis. Cross-sections of the intestine from animals at premetamorphic stage 54 (A, E), metamorphic climax stages 61 (B, F) and 62 (C, G, I, J, O, Q), and the end of metamorphosis (stage 66; D, H), and stage-54 tadpoles treated with 10 nM T3 for 3 days (K, M) and 5 days (N, L, P, R) were hybridized with antisense Hairy1 (A-D, K, L, O, P), Hairy2b (E-H, M, N, Q, R), or their sense probes (I, J). Dark blue deposits indicate the sites of probe binding. Light or dark brown pigments in some pictures are melanin. Hairy1 is expressed in the epithelium (Ep), whereas Hairy2b is expressed in the connective tissue (CT). The boxed area in panel A is magnified to show the localization of Hairy1 expression in the larval Ep (panel A, inset). The boxed areas in panels C, G, L, N are magnified and shown in panels O, Q, P, R, respectively. The dashed-lines indicate the boundary of Ep and CT. Scale bars = 100 μm (A-L), 20 μm (inset of A, O-R). Abbreviation: CT, connective tissue, Ep, epithelium. Image published in: Hasebe T et al. (2017) © 2016 The Authors STEM CELLS published by Wiley Periodicals, Inc. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives license Larger Image Printer Friendly View

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