Xenbase Image ID: 154236

Fig. 6. Nlk1 kinase activity is dispensable for gastrulation regulation and PAPC stabilization. A, Quantification of gastrulation defects in stage 35 Xenopus embryos injected dorsal-equatorially with indicated mRNA (250 pg/embryo). Representative phenotypes are shown on the right. B, ATF2 luciferase reporter assay in stage 12 embryos injected with indicated mRNA (wnt11 and lower dose of nlk1 and nlk1 kn: 250 pg/embryo, higher dose: 500 pg/embryo). The graph shows the mean±SD of three independent experiments (** p>0.01 compared with Wnt11 induced control). RLU: relative light units. C, Immunoblot analysis of lysates from stages 10 gastrula embryos injected with the indicated MOs (12 ng/embryo) or mRNA (300 pg/embryo). GFP and GAPDH were used as transfection and loading controls. D, Immunofluorescence microscopy of AC explants. Embryos were injected animally at the four-cell stage with 300 pg/embryo of nlk1 kn-ha (green) and the indicated papc-myc constructs or mbRFP as control (red). Nuclei were counterstained with DAPI (blue). Scale bar=50 µm. White arrowheads indicate co-localization at the plasma membrane and the asterisk marks expression of both proteins inside the nucleus. Image published in: Kumar R et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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