Xenbase Image ID: 154239

Supplementary Fig 3. Validation of the custom-made anti-PAPCc antibody. A, Immunoblot analysis of embryo lysates from indicated developmental stages, and stage 10 gastrula embryo lysates injected with the indicated MOs (12 ng/embryo) or mRNAs (300 pg/embryo). Overexpression of PAPC-Myc was confirmed using an anti-Myc antibody. GFP and GAPDH were used as injection and loading controls, respectively. B, Immunofluorescence staining of AC explants overexpressing PAPC or M-PAPC (300 pg mRNA/embryo). PAPC was detected using the custom anti-PAPCc antibody (green). Arrowhead shows PAPC protein at the membrane visualized by co-injection of mbRFP (red). DAPI (blue) was used for nuclear staining. Scale bar=50 µm. Image published in: Kumar R et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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