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Supplementary Fig 4. GSK-3β reduced PAPCc protein levels. A, Immunoblot analyses of overexpressed PAPCc in HEK293 cells. Cells were transfected with indicated plasmids and treated with 10 µm MG132 or 10 mM LiCl for 4 h prior to protein extraction. NaCl was used as negative control. GFP and GAPDH were used as transfection and loading controls, respectively. B, Immunoblot analysis of PAPCc levels in HEK293 cells following concomitant overexpression of GSK-3β. GFP and β-Tubulin were used as transfection and loading controls. Image published in: Kumar R et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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