Xenbase Image ID: 154320

Figure 4. Constitutive Cre activity in Xenopus embryos. (A) The Cre reporter construct CMV:GFP(loxP)lacZ is schematically drawn with the CMV promoter/enhancer driving the expression of the GFP and lacZ genes. The position of the SV40 EpA and LpA signals as well as the target sites of the Cre recombinase (loxP) are given. (B and C) Stage 38 Xenopus larvae were derived from eggs injected with CMV:GFP(loxP)lacZ DNA (B) or coinjected with CMV:GFP(loxP)lacZ DNA and Cre mRNA (C). The living animals were photographed in normal light (top) or in fluorescence light (middle). X-gal staining was obtained after fixation of the larvae (bottom). Image published in: Werdien D et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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