Xenbase Image ID: 154399

Figure 4. A dominant negative XTbx1 interferes with Xenopus development. Embryos were injected with XTbx1-enR mRNA into both blastomeres at the two-cell stage, then fixed and photographed at stage 35. Embryos are orientated where possible with the dorsal side uppermost and anterior to the left. A: Injection of a total of 20 pg of RNA results in mild defects that primarily affect anterior structures, including reduced eye and head and pericardial edema. B: Injection of 200 pg of RNA leads to more severe loss of anterior structures in many embryos. Defects also become apparent after the onset of gastrulation, leading to a drastic foreshortening of the anterior–posterior axis. C: Injection of 1 ng of RNA leads to the most severe defects, which predominantly become apparent during gastrulation. Embryos may develop rudimentary eyes or cement glands and have edematous, malformed hearts. Image published in: Ataliotis P et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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