Xenbase Image ID: 154404

Figure 9. Fgf8 expression is dependent on XTbx1. Embryos were injected in one blastomere at the two-cell stage with 250 pg of XTbx1-enR and 250 pg of β-galactosidase. Embryos were fixed at stage 32 and processed for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) staining, then re-fixed and processed for in situ hybridization with a probe for fgf8. A:fgf8 transcripts were detected in two broad stripes in the branchial arches on the uninjected side. B: On the injected side of the embryo, fgf8 staining is visible in the dorsal region of the caudal stripe but diminishes in the ventral region (arrowheads) where X-gal–stained cells are present. fgf8 expression in the rostral stripe is further diminished, and there is a region between the branchial arches that does not express fgf8, in contrast to the uninjected side. Image published in: Ataliotis P et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue fgf8.L AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 X. laevis Throughout NF stage 32 branchial arch Image source: Published Larger Image Printer Friendly View

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