Xenbase Image ID: 154544

Supplemental Fig. 3. Examples of fragment analysis, western blot, and immunohistochemistry for various genes tested. FA genotype – X-axis=size of amplicon in basepairs (bp), Y-axis=relative intensity of PCR signal to loading control. (a–e) Fragment analysis of embryos injected with Cas9 protein+sgRNA – galnt11, dnah9, ccdc40, beta-catenin, pax8. (f) Western blot analysis using antibody against Foxj1. Quantification of changes in protein level was normalized to GAPDH and calculated using ImageJ software from NIH. (g) Immunohistochemistry of stage 28 embryos with acetylated tubulin to mark surface cilia. Left to right – WT, dnah9, ccdc40, and galnt11. Image published in: Bhattacharya D et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xtr Wt + ccdc40 CRISPR + NF28 (immunohistochemistry) Table. 1,Fig. S3.g Anatomical Phenotype abnormal ciliated epidermal cell Disease primary ciliary dyskinesia 15 Xtr Wt + dnah9 CRISPR + NF28 (immunohistochemistry) Table. 1,Fig. S3.g Anatomical Phenotype abnormal heart looping Xtr Wt + galnt11 CRISPR + NF28 (immunohistochemistry) Table. 1,Fig. S3.g Anatomical Phenotype abnormal heart looping Larger Image Printer Friendly View

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