XB-IMG-154581
Xenbase Image ID: 154581
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Figure 1
Comparative expression pattern of hairy genes. Whole-mount in situ hybridization analysis of spatio-temporal hairy genes expression. A–C, E–G, I–K, M, and O–S: Dorsal view of Xenopus laevis embryos; anterior side is on the right. N: Dorso–lateral view. D, H, L: Lateral view. M′, N′, O′,Q′, R′: Transversal sections. Dashed lines in M, N, O, P, Q and R indicate the sites of transverse section shown in M′, N′, O′, P′ Q′ and R′, respectively. A: hairy1 transcripts are first detected since the early neurula stage (stage 11.5) in the dorsal region of embryos at the neural plate (black arrowheads) and neural plate borders (white arrowheads). B,C: During neurulation (stages 13–16), hairy1 is expressed in the neural plate borders (arrowheads) and surrounding the anterior neural plate (asterisk). At stage 16 the increased expression in the anterior neural plate is remarkable. D: Stage 20 embryo. Arrowheads show the expression from the dorsal to the anterior–ventral region. The red arrowhead shows the anterior expression in the optic vesicle. Arrowheads show the expression in different placodes (see text). E: hairy2a transcripts are first detected since the early neurula stage (stage 11) in the dorsal region of embryos at the neural plate border (yellow and black arrowheads) and in the prechordal mesoderm (white arrowheads). F,G: During neurulation (stages 13–16), hairy2a expression is predominant in the anterior neural fold (yellow arrows), in the neural crest region (black arrowheads) and in the prospective floor plate (white arrowheads). H: Stage 22 embryo. The expression of hairy2a can be seen in the cephalic streams of migrating neural crest cells (black arrowheads) and in the optic placode (red arrowhead). I: Early expression of hairy2b can be observed in the anterior neural fold (yellow arrow), in the lateral neural fold (black arrowhead) and in the posterior part or the neural plate (white arrowhead). J,K: During neurulation (stages 13–16), the expression of hairy2b can be observed in the prospective neural crest region (black arrowheads), in the anterior neural fold (yellow arrowhead), in the prospective floor plate (white arrowheads), and a transversal expression in the middle of the neural plate (green arrowhead). L: Stage 21 embryo. hairy2b expression is shown in migratory cephalic neural crest (black arrowheads) and the optic placode (red arrowhead). M–R: Double in situ hybridizations for hairy and foxd3, or sox2. M′–N′: Transverse sections of St. 15 neurula embryos displayed in M–N showing that hairy1 (M′, purple, arrowheads) expression is surrounded by foxd3 (neural crest expression, turquoise, asterisk), and hairy1 expression (N, purple) is located at the outer limit (arrowheads) of the neural plate domain revealed by sox2 gene expression (turquoise, white asterisk). O′–R′: Transverse sections of St. 15 neurula embryos displayed in O–S showing that hairy2a and hairy2b (turquoise) expression overlaps with foxd3 (purple) in the neural crest territory (O′ and R′, black asterisk: foxd3 neural crest expression) and is complementary with sox2 (turquoise) expressions (P′,R′). np, neural plate. Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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