Xenbase Image ID: 154589

Figure 6 hairy genes participate in the early formation of neural crest cells. A–X: Dorsal views of Xenopus laevis embryos; anterior side is on the right. The injected side is indicated by an arrowhead. Embryos were injected into one blastomere at the 8- to 16-cell stage with 700 ng of mRNA, treated with dexamethasone stage 11 until neurula stages and fixed, and the expression of several markers was analyzed by in situ hybridization. No change was observed in the control injected embryos nonincubated with dexamethasone (insets in A, E, I, M, Q, and U). A–L: Gain of function. hairy activator constructs mRNA-injected embryos show increased expression of snail2 (A,E,I) while neural plate marker sox2 (B,F,J) and epidermis marker xk81a (C,G,K) are reduced. D,H,L: Embryos labeled by double in situ hybridization for sox2 and xk81a genes showing the increase in prospective neural crest region (black brackets) in the injected side (arrowheads). M–X: Loss of function. Conversely, DNhairy constructs mRNA-injected embryos show that neural crest markers snail2 are reduced in the injected side (M,Q,U) while neural plate marker sox2 (N,R,V) and the epidermis marker xk81a (O,S,W) are slightly increased. P′, T′, X′: Rescue of DNhairy by coinjecting mRNA of hairy activator constructs. The phenotype of the neural crest marker foxd3 was rescued by the co-injection of hairy1-GR (P′), hairy2a-GR (T′) and hairy2b-GR (X′). P,T,X: Embryos labeled by double in situ hybridization for sox2 and xk81a genes showing the decrease in prospective neural crest territory (black brackets indicate width) in the injected side. Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Sometime during NF stage 15 to NF stage 16 neural crest cranial neural crest pre-chordal neural plate border neural plate border sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Sometime during NF stage 15 to NF stage 16 neural plate krt12.4.L ck81, cytok, cytokeratin, E-keratin, epi-keratin, epidermal keratin, Epidermis-specific keratin, EpiK, epiker, EpK, epK, K81, xK81, xk81a, xk81a1 X. laevis Sometime during NF stage 15 to NF stage 16 non-neural ectoderm epidermis foxd3.L fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 X. laevis Sometime during NF stage 15 to NF stage 16 neural crest cranial neural crest pre-chordal neural plate border neural plate border Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + hes1-GR + DEX + NF16 (in situ hybridization) fig.6.a Expression Phenotype increased amount snai2.L expression in neural crest Xla Wt + hes1-GR + DEX + NF16 (in situ hybridization) fig.6.b,d Expression Phenotype decreased amount sox2.L expression in neural plate Xla Wt + hes1-GR + DEX + NF16 (in situ hybridization) fig.6.c,d Expression Phenotype decreased amount krt12.4.L expression in epidermis Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.e Expression Phenotype increased amount snai2.L expression in neural crest Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.f,h Expression Phenotype decreased amount sox2.L expression in neural plate Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.g,h Expression Phenotype decreased amount krt12.4.L expression in epidermis Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.i Expression Phenotype increased amount snai2.L expression in neural crest Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.i, l Expression Phenotype decreased amount sox2.L expression in neural plate Xla Wt + hes4-GR + DEX + NF16 (in situ hybridization) fig.6.k, l Expression Phenotype decreased amount krt12.4.L expression in epidermis Xla Wt + dnhes1-GR + DEX + NF16 (in situ hybridization) fig.6.m Expression Phenotype decreased amount snai2.L expression in neural crest Xla Wt + dnhes1-GR + DEX + NF16 (in situ hybridization) fig.6.n, p Expression Phenotype increased amount sox2.L expression in neural plate Xla Wt + dnhes1-GR + DEX + NF16 (in situ hybridization) fig.6.o, p Expression Phenotype increased amount krt12.4.L expression in epidermis Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.q Expression Phenotype decreased amount snai2.L expression in neural crest Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.r, t Expression Phenotype increased amount sox2.L expression in neural plate Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.s, t Expression Phenotype increased amount krt12.4.L expression in epidermis Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.u Expression Phenotype decreased amount snai2.L expression in neural crest Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.v, x Expression Phenotype increased amount sox2.L expression in neural plate Xla Wt + dnhes4-GR + DEX + NF16 (in situ hybridization) fig.6.w, x Expression Phenotype increased amount krt12.4.L expression in epidermis Larger Image Printer Friendly View

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