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Figure 8 hairy2a activity on naive ectodermal tissue. Each blastomere of a 2-cell stage embryo was injected with 1 ng of inducible construction mRNA of hairy2aGR, msx1GR or a mixture of both genes. The embryos developed until stage 8, and then animal caps were isolated and incubated with dexamethasone from equivalent stage 11 until stage 20. Total mRNA was isolated and analyzed by RT-PCR. A: The expression of ectodermal tissue markers sox2 (neural plate) and snail1 (neural crest) was assessed. The expression of ef1α was analyzed as a loading control for each sample. B: Quantitation of gene expression results shown in panel A. The results are expressed as relative intensity values (calculated as the ratio between each sample band intensity and its corresponding ef1α band intensity × 100). Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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