XB-IMG-154592
Xenbase Image ID: 154592
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Figure 9
hairy genes participate in the control of apoptosis during neural crest initial development. A–H: Dorsal views of Xenopus laevis embryos; anterior side is on the right. The injected side, indicated by an arrowhead, is also recognizable by the presence of the lineage tracer FDx (turquoise). Wholemount TUNEL labeling was performed as indicated in the Experimental Procedures section. Embryos were injected into one blastomere at the 8- to 16-cell stage with 700 ng of hairy inducible constructs mRNA (A–C), DNhairy constructs mRNA (D–F), hairyMO (G), or ControlMO (H), incubated until stage 17 and fixed. For inducible constructs, dexamethasone was added at stage 14, after the initial specification of neural crest. I: Quantification of TUNEL labeling for hairy construction-microinjected embryos (see Experimental Procedures section). The changes in apoptosis status caused by the microinjection of hairy constructions mRNA were statistically significant (Student t-test: **P ≤ 0.05; *P ≤ 0.1). Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.
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