Xenbase Image ID: 154594

Figure 11 hairy genes are differentially required for the neural crest migration process. The anterior side of the embryos is on the right. One blastomere of a 8- to 16-cell stage embryo was injected with mRNA encoding inducible constructions of hairy2 genes and were cultured until stage 17 or 20 when the construction was activated with dexamethasone. After activation, embryos were cultured until stages 21 or 23, respectively, and the expression pattern of the snail2 marker was analyzed. Black arrowheads indicate the injected side, which can be also recognized by the turquoise FDA staining. A,C: The overexpression of the dominant negative inducible constructs markedly decreased the migration of cephalic neural crest cells when dexamethasone was added at the beginning of neural crest cell migration (stage 17), in comparison with the control side (A′–D′). B,D: Neural crest cephalic migration was slightly inhibited when DNhairy2 inducible constructs were activated at stage 20 when the migration was occurring. The leading edge of cephalic streams migration is indicated by a dashed line. E–H: Co-injection of DNhairy2 mRNA and mRNA encoding a different hairy2 gene inducible construct. E,G: The effects of reduced migration of DNhairy2a on the expression of the ectodermal marker snail2 were rescued by the co-injection of hairy2b. F,H: The effects of reduced migration of DNHairy2b on the expression of the ectodermal marker snail2 were partially rescued by directed co-injection of hairy2a. Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Sometime during NF stage 17 to NF stage 23 neural crest cranial neural crest migratory neural crest cell premigratory neural crest cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + dnhes4-GR + DEX + NF21-22 (in situ hybridization) fig.11.a Anatomical Phenotype abnormal cell migration in neural crest Expression Phenotype decreased amount snai2.L expression in cranial neural crest Xla Wt + dnhes4-GR + DEX + NF21-22 (in situ hybridization) fig.11.b Anatomical Phenotype abnormal cell migration in neural crest Expression Phenotype decreased amount snai2.L expression in cranial neural crest Xla Wt + dnhes4-GR + DEX + NF21-22 (in situ hybridization) fig.11.c Anatomical Phenotype abnormal cell migration in neural crest Expression Phenotype decreased amount snai2.L expression in cranial neural crest Xla Wt + dnhes4-GR + DEX + NF21-22 (in situ hybridization) fig.11.d Anatomical Phenotype abnormal cell migration in neural crest Expression Phenotype decreased amount snai2.L expression in cranial neural crest Larger Image Printer Friendly View

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