Xenbase Image ID: 154596

Figure 13 hairy genes are involved in the formation of craniofacial cartilage derived from neural crest. Embryos were injected in one blastomere at the 16-cell stage; cartilage was visualized after Alcyan-blue staining and analyzed at stage 45. A,B,D: Anterior part is toward the top, ventral view. Arrowheads indicate the injected side. At stage 45, hairy2bMO-injected (B) embryos show a reduction in cranial cartilage while ControlMO-injected (A) embryos show no changes in the same cartilage. C: Diagram of the effects of hairy2bMO on head cartilage of a Xenopus tadpole. CH, ceratohyal cartilage; CB, ceratobranchial cartilage; BH, basihyal cartilage; Ir, infrarrostral; M, Meckel cartilage. E–H: Normal cartilage formation was completely rescued by co-injection of hairy2bMO and hairy1-GR mRNA. E,F: Analysis of melanocyte (Stage 38) formation in embryos injected with 700 ng of hairy2b-GR (E,G) and DNhairy2b-GR (F,H) mRNA. Injected embryos do not show inhibition in melanocyte development. Image published in: Vega-López GA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Experiment + Assay Source Phenotypes and Disease Xla Wt + hes4 MO + NF45 (stained tissue microscopy) fig.13.b Anatomical Phenotype abnormal development of craniofacial region abnormally decreased number of ceratohyal absent branchial arch skeleton decreased size of the Meckel's cartilage Larger Image Printer Friendly View

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