XB-IMG-154609
Xenbase Image ID: 154609
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Figure 5.
A: Schematic showing the steps in the cell spreading assay. B: 24 hr after explanting epidermis and treating it with DMSO (control). C: 24 hr after explanting epidermis and treating it with the JNK inhibitor. D: Schematic showing the treatment paradigm for the EGTA adhesion assay. E: Control embryo treated with DMSO only. Inset shows E-cadherin labeling confined to edges of cells. F: Representative embryo treated with DMSO followed by EGTA. White arrows point toward regions where the tissue is dissociating. Inset shows internalized E-cadherin. G: Embryo treated with JNK inhibitor alone. Inset shows E-cadherin labeling confined to cell boundaries. H: Embryo treated with JNK inhibitor followed by EGTA. Inset shows E-cadherin largely confined to the cell boundaries. I: The epidermis of a representative embryo showing the cells containing fluorescein labeled Control MO (green) and E-cadherin labeled in red. I′: Corresponding image from I showing only the E-cadherin labeling in red. I′: Corresponding image from I showing only the E-cadherin labeling in black and white. Insets show representative 1–2 cells at 2 × magnification. J: The epidermis of a representative embryo showing the cells containing fluorescein labeled JNK1 MO (green) and E-cadherin labeled in red. J′: Corresponding image from J showing only the E-cadherin labeling in red. J′: Corresponding image from J showing only the E-cadherin labeling in black and white. Insets show representative 1–2 cells at 2 × magnification. Black arrows indicate groups of cells with higher levels of E-cadherin at the membrane. K–L: E-cadherin labeling of epidermis from representative embryos (stage 24–26) that were uninjected (K,K′) or injected with CA-JNK (L,L′). Prime letters are the identical pictures in black and white. Insets show a two-fold magnified image of a cell from the images. White arrows show what appears as puncta of E-cadherin positive bodies within the cytoplasm and arrowheads point to membranes with reduced E-cadherin. Insets show representative 1–2 cells at 2 × magnification. M–N: Clathrin labeling of epidermis from representative embryos (stage 24–26) that were uninjected (M,M′) or injected with CA-JNK (N,N′). Prime letters are the identical pictures in black and white. Insets show representative 1–2 cells at 2 × magnification. White arrows indicate nonspecific staining where fluorescence is observed on top of the cell. Image published in: Houssin NS et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons.
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