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Xenbase Image ID: 154779

Fig. 3. Noggin + FGF generates neural tissue with anterior-posterior polarity. Ectoderm from stage 10.25 embryos was treated with increasing doses of bFGF (2 ng/ml, 10 ng/ml, 50 ng/ml) either in the absence (A-C) or presence (D-F) of 1 mg/ml noggin. Explants were cultured until control whole embryos (G) reached the late neurula stage (stage 18). Expression of otx2 and Hox B9 were analyzed by in situ hybridization. Otx2 (light blue) is expressed at the anterior end, and Hox B9 (purple) is expressed at the posterior end of the control embryo (G; the dark color at the anterior end is an effect of the embryo edge, and is not Hox B9 staining). Increasing bFGF dose increases Hox B9 expression (A-C). In the presence of noggin, increasing bFGF dose(D-F) causes the explants to elongate and to express otx2 and Hox B9 on either end of the elongated structure. Expression of a general neural marker, nrp-1 (purple) and muscle actin (blue) was examined in whole embryos (H) and in explants treated with 1 mg/ml noggin (I), 50 ng/ml bFGF (J), and the combination of 1 mg/ml noggin and 50 ng/ml bFGF (K). Noggin alone induces nrp-1 expression, bFGF alone induces both muscle actin and nrp-1, and the combination of the two induces only nrp-1, no muscle actin expression is observed.

Image published in: Lamb TM and Harland RM (1995)

Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

nrp1.LA5 antigen, np-1, neuropilin-1, nrp, npn-1, nrp-1, neuropilin, pNPG152X.laevisThroughout NF stage 20neural tube
otx2.LXotx2, otx-2, Xotx-2, otxA, otx2-a, otx2-bX.laevisThroughout NF stage 20anterior
hoxb9.Lxhoxb9, hox 2.5, XlHbox-6, XlHbox6X.laevisThroughout NF stage 20posterior
actc1.Lcardiac actin, actc, cardiac alpha actin, actna1, muscle actinX.laevisThroughout NF stage 20somite

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