Xenbase Image ID: 154793

Figure 2. Schematic of homology-independent strategy for targeted integration in X. tropicalis. Upon coinjection of Cas9 mRNA, gRNA, and circular donor DNA into X. tropicalis fertilized eggs at animal pole, chromosomal target site and donor plasmid would be simultaneously cleaved in vivo. Resultant DNA ends would be ligated by error-prone nonhomologous repairs (NHRs). If desired, in principle, the vector backbone and the selection cassette (ela:EGFP) can be removed with the Cre/LoxP system. GOI, gene of interest; pA, SV40 polyadenylation signal Image published in: Shi Z et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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