Xenbase Image ID: 154809

FIG. 2. Specific binding of iodinated BMP-4 to COS cells transfected with mTFR11. (A) 'MI-BMP4 binding to COS cells transfected with mTFR11. Binding was performed on cell monolayers as described below and was competitively inhibited with unlabeled BMP-4. (Inset) Scatchard analysis. o, pMV2; *, mTfr11/pMV2. (B) Specificity of mI-BMPA binding to COS cells transfected with mTFR11. Binding of 125I-BMP4 to COS cells transfected with mTFR11 was performed with or without increasing concentrations ofunlabeled ligands. *, BMP-2; o, BMP-4;m, activin A; *, TGF-.l1. (C) Chemical cross-linking ofiodinated BMP-4 to COS cells transfected with mTFR11/pMV2 orpMV2. COS cells were transfected with either pMV2 (lane 1) or mTFR11/pMV2 (lanes 2-5) and incubated with 5 ng of 125I-BMP-4 per ml (all lanes) and 500 ng ofBMP-4 per ml (lane 3), 500 ng of BMP-2 per ml (lane 4), or 500 ng of activin per ml (lane 5). Molecular masses are indicated in kDa. Image published in: Suzuki A et al. (1994) Copyright © 1994. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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