Xenbase Image ID: 154843

Figure 6. miR-182 Is Required for Slit2-Induced Local Translation of Cfl1 in RGC GCs (A) Schematic representation of the experimental protocol. After 24 hr, RGC axons were isolated from their cell bodies. Bath application of Slit2 at a suboptimal concentration was used to avoid collapse. Vehicle was used as control. Recovery of the newly synthesized Kaede green protein was monitored over time. (B) Quantification of the recovery of Kaede green signal. Data are presented as the percentage change of the fluorescence intensity (F) over time. Numbers of GCs analyzed are indicated in the legend of the graph. (C and D) Representative pre- and post-photoconversion images of severed control (C) or miR-182 morphant (D) axons. Values are mean ± SEM (B). Kruskal-Wallis test, ∗p < 0.05, ∗∗p < 0.01. Scale bars, 10 μm (C and D). Cont, control; LPS, local protein synthesis; MO, morpholino oligomer. See also Figure S6. Image published in: Bellon A et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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