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Figure S4: MO-mediated Slit2 knockdown affects the size of the eye, Related to Figure 4. (A) Representative images of stage 40 embryos whose dorsal blastomeres have been injected with control or Slit2 MOs at 8 cell stage. (B-C) The efficiency of Slit2 knockdown was assessed by Western Blot. (B) Representative western blot for Slit2 and α-tubulin of head lysates from stage 40 control or Slit2 morphants. (C) Quantification of Slit2/ α-tubulin intensity ratio (n=3) shows a drastic reduction of Slit2 expression in the presence of Slit2 MO in the nervous system. Values are mean ± SEM. Wilcoxon paired test, **p<0.01. ns, nonsignificant; cont, control; MO, morpholino. Scale bar, 1mm (A). Image published in: Bellon A et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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