Xenbase Image ID: 154850

Figure S5: miR-182 is not required for Sema3A-induced repulsive turning of RGC axons in vitro, Related to Figure 4. In vitro turning assay on stage 35/36 RGC axons cultured for 24h and isolated from their cell bodies. (A) Representative images of control or miR-182 morphants RGC GC before and 60 minutes after being exposed to a gradient of Sema3A established from a pipette (top right corner) set at 45° angle from the initial direction of growth. Scale bar, 30µm. (B) Tracings of RGC axons analyzed. Source of guidance cue is indicated by arrowhead. Red, black and blue traces represent, respectively repulsive behaviors (angle <-5°), nonsignificant changes in the direction of growth (-5°5°). (C) Quantification of the average turning angle ± SEM. Numbers of GCs analyzed are between brackets. Mann-Whitney test, **p<0.01. ns, nonsignificant. Image published in: Bellon A et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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