Xenbase Image ID: 154923

Figure 2. Leftward Flow Is Required for nodal1 Expression in Conjoined Twins (A) Injection scheme and predicted nodal1 expression in CoMO- or dnah9MO-injected embryos following βcat-induced twinning on the left (iL) or right (iR) side. (B–D) Left-sided nodal1 expression (closed arrowhead) in CoMO-injected twins (B) was absent (open arrowhead) in dnah9-injected specimens upon twinning on the right (C) but present (closed arrowhead) when the induced twin was placed on the left of the endogenous embryo (D). (E) Summary of nodal1 expression in the induced twin (green, present; red, absent; n, number of analyzed specimens). ∗∗∗p < 0.001; ns, not significant. (F–L) nodal1 (F and G) and dand5 (H–L) expression in twinned GRPs of flow (F and H) and post-flow (G and I–L) embryos. Arrowheads, non-reduced dand5; open arrowheads, reduced dand5. Note that dand5 was clearly reduced on the left side of the left embryo (open arrowhead) but only partially in the left half of the fused central domain. ∗, induced twin. See also Movie S3. Image published in: Tisler M et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ctnnb1 + dnah9 MO + NF22 (in situ hybridization) Fig 2 B Expression Phenotype decreased amount nodal1.L expression in lateral plate mesoderm Larger Image Printer Friendly View

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