Xenbase Image ID: 154926

Figure S1 (Related to Figure 1) Nodal cascade expression and organ situs in ßcat-induced conjoined twins (A) Injection scheme, observed Nodal cascade expression and heart situs in twinned embryos. ßcat mRNA injection into the left (L) or right (R) blastomere of the 4-cell embryo induced twinning on the L (iL) or R (iR) side of the primary embryo. (B-D) Representative specimens displaying nodal1 (B), lefty2 (C) and pitx2c (D) in the left LPM of the left twin. (E-G) Heart looping in ßcat-induced conjoined twins. Representative stage 45 tadpoles displaying situs solitus in the left and situs inversus in the right twin (E, blowup in F), or situs solitus on both sides (G). (H-J) Summary of nodal1 and pitx2c expression patterns in the left LPM of the left twin in iL and iR specimens of ßcat- (H), wnt8a- (I) and sia1-induced (J) conjoined twins, as well as compilation of heart situs data. Note that the Nodal cascade was only induced on the left side of the left twin, irrespective of the placement of the induced twin. Note also that the heart situs was normal in left twins and randomized in right ones, again irrespective of the placement of the induced twin. LT, left twin; RT, right twin. Image published in: Tisler M et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ctnnb1 + NF45 (whole-mount microscopy) Fig S1 E F G Anatomical Phenotype abnormal heart looping Xla Wt + ctnnb1 + NF45 (whole-mount microscopy) Fig S1 H-J Anatomical Phenotype abnormal determination of left/right symmetry abnormal nodal signaling pathway Larger Image Printer Friendly View

Return to previous page