Xenbase Image ID: 154978

Figure 1. Microtubule Organization around Wound Borders All times are in min:sec. (A) Confocal image of a laser-wounded oocyte. Microtubules (red), F-actin (green), and myosin-2 (blue) show complementary distribution around the wound. (B) Individual channels (MT, microtubule; FA, F-actin; and M2, myosin-2). F-actin and myosin-2 concentrate inside the area of highest microtubule density. (C) Image from a movie of an OG-Tax-injected oocyte. Microtubules are organized into a radial array around the wound (W). See Movie 1 (Movies are available in the Supplemental Data available with this article on line). (D) Image from a movie of a wound in an OG-Tax-injected oocyte. Microtubules are perpendicular to the wound border and translocate to the wound. See Movie 2. (D ) Time course of microtubule movement toward the wound (indicated by an arrow). Asterisks mark the leading ends of individual microtubules, and arrowheads mark the trailing ends. (E) Kymograph of OG-Tax-labeled microtubule motility toward the wound edge. Horizontal arrow is 45 s; vertical arrow is 5 m. Microtubules accelerate as they approach the wound border (w), then slow at its immediate edge. (F) Images from a movie of an OG-Tax-injected oocyte. An arrowhead shows a sharply bent microtubule moving toward the wound. (G) Images from a movie of an OG-Tax-injected oocyte. Microtubules buckle (arrowhead) at the wound border. The arrow indicates direction of flow. Image published in: Mandato CA and Bement WM (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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