Xenbase Image ID: 154979

Figure 2. Microtubule Transport is F-Actin Dependent (A) Plot of instantaneous rates of microtubules before and after treatment with latrunculin B (20 M). Microtubule translocation to the wound border was arrested after latrunculin treatment. For each point, the rates of movement of a minimum of 10 microtubules were quantified; bars indicate standard error of the mean (SEM). (B) Images from a movie of an OG-Tax-injected oocyte. Arrowheads show a microtubule translocating to the wound (W), until latrunclin application (asterisk). See Movie 3. (C) Double-labeled image from a movie of an oocyte injected with OG-Tax (green) and Alexa 568-phalloidin (red). See Movie 5. (C ) Microtubules move to the wound (arrow) in association with F-actin and buckle upon reaching the wound edge (arrowheads). See Movie 6. (D) Dual-label images showing end-end association between F-actin (FA, red) and microtubules (MT, green); the overlap of the two is indicated in two frames by arrowheads. F-actin is localized to the tip of microtubules moving to the wound. The horizontal arrow is 30 s; the vertical arrow is 15 m. See Movie 7. (E) Dual-label image from a movie showing microtubules (green) aligned with F-actin (red; alignment indicated by arrowheads). Image published in: Mandato CA and Bement WM (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page