Xenbase Image ID: 154980

Figure 3. Cotransport of F-Actin and Microtubules (A) Dual-label images showing examples of bent microtubules. Arrows indicate direction of movement. Microtubules (green) bend away from the direction ofmovement where they are not associated with F-actin (red). - (B) Images from a movie showing F-actin (red) and microtubules (green). F-actin appears to link the ends of several microtubules and drag them through the cortex. See Movie 8. (C) Dual-label and split-channel images showing buckling and breakage of a microtubule that is associated with F-actin at both leading and trailing edges. F-actin at the trailing edge accelerates between the 00:45 and 01:00 time points, whereas the F-actin at the leading edge does not (white lines). Microtubule buckling is observed in precisely this time window. Later (at 01:30), the microtu- bule breaks (arrow). See Movie 9. (D) Quantification of specific microtubule-F-actin overlap (see Supplemental Data for details). The ratio of yellow pixels in unrotated: rotated images is significantly higher in the region where microtu- bule-F-actin cotransport occurs (10–30 m) than at the wound edge (0–10 m) or in areas more than 30 m away from wound. Results are mean  SEM from four independent experiments; an asterisk indicates  0.01. (E) Double-labeled kymograph. F-actin (red) and microtubules (green) show same patterns of acceleration and deceleration. W indicates the wound; the horizontal arrow is 90 s; the vertical arrow is 10 m. Image published in: Mandato CA and Bement WM (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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