XB-IMG-154981
Xenbase Image ID: 154981
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Figure 4. Local Actin Assembly Coincides with Microtubule Breakage and Assembly
(A) Image from a movie of an oocyte injected with OG-taxol and Alexa 568 G-actin. Microtubules (green) move to the zone of actin polymerization
(red; indicated by a double-headed arrow). Microtubule disappearance into the zone is correlated with bending and buckling. See Movie 10.
(B) Dual-label image from a movie showing microtubules translocating toward the wound (an arrow indicates the direction of the wound).
Arrowheads show individual microtubules buckling.
(C) Plot of microtubule buckling and relative actin intensity versus distance from the wound border. Microtubule buckling is most common
near the actin assembly zone.
(D) Image showing e-GFP-Xtub-labeled microtubules (arrowheads) near the wound.
(E) Images from a movie of an oocyte injected with e-GFP-Xtub. Microtubules polymerize perpendicular to (left panels) and away from the
wound (right panels). Arrows indicate the direction of flow; arrowheads show the start and stop of polymerization; frames are shown at 4 s
intervals. See Movies 11 and 12.
(F) Graph showing that microtubule polymerization rates are inversely correlated with distance from the wound.
(G) Plot of microtubule polymerization events and relative actin intensity versus distance from the wound border. Microtubule assembly events
are most common near the zone of actin assembly.
(H) Image from a movie of an oocyte wounded after injection with e-GFP-X tub and treated with latrunculin B (20 M). Microtubules accumulate
around the wound border. Image published in: Mandato CA and Bement WM (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |