XB-IMG-154983
Xenbase Image ID: 154983
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Figure 6. Microtubule Perturbation Disrupts
Normal Patterns of Arp2/3 Complex and Myosin-
2 Localization and Uncouples the Zone
of Actin Polymerization from the Contractile
Ring
(A) Confocal images showing distribution of
p16 around wounds in control and taxoltreated
oocytes. The region of p16 accumulation
is broader in the taxol sample. Quantification
shows that the width of the region
enriched in p16 increases in the presence of
taxol. Results are mean SEM from three
independent experiments; an asterisk indicates
p 0.01.
(B) Confocal images showing examples of
taxol-induced spreading (Taxol-Spread) or
splitting (Taxol-Split) of the region of myosin-
2 enrichment around wounds and, for
comparison, controls. Quantification shows
that the width of the region enriched in actin
and myosin-2 around wounds increased in
the presence of taxol. Results are mean
SEM from three independent experiments; an
asterisk indicates p 0.01.
(C) Double-labeled images from 4D movies
of oocytes injected with Alexa 568-G-actin
and OG-phalloidin, treated with taxol (taxol)
or nothing (control), and wounded. Lower
panels show images taken from the regions
indicated by the arrows in the upper image.
In control, the zone of assembly (red) encompasses
the contractile ring that contains stable
F-actin (green). However, in the taxol sample,
dynamic actin moves to the outer ring,
whereas stable F-actin remains concentrated
at the wound border. See Movies 16 and 17. Image published in: Mandato CA and Bement WM (2003) Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |