Xenbase Image ID: 155100

Figure 2. WNT9B activates PKD1/TRPP2Time course of WNT9B-induced Δ[Ca2+]i (shown as fluorescence ratio 340/380) in untransfected (a, red, n=52 cells pooled from 3 independent experiments) or PKD1/TRPP2 co-transfected CHO-K1 cells (b, red, n=30 cells pooled from 5 independent experiments) in 1.8 mM extracellular Ca2+. Ionomycin (I, 2 μM) was added at time point 35 min. (c) Time course of WNT9B-induced Δ[Ca2+]i in PKD1/TRPP2 co-transfected CHO-K1 cells (red, n=65 cells pooled from 5 independent experiments) in Ca2+ free extracellular solution (10 μM EGTA). Δ[Ca2+]i in PBS-treated cells in all three conditions is shown in black. (d-l) Time course (d-f), step currents (g-i), or I-V curves of WNT9B (500 ng/ml)-induced whole cell currents in untransfected (d,g,j), PKD1/TRPP2 co-transfected (e,h,k) or PKD1S99I/TRPP2-co-transfected CHO-K1 cells (f,i,l) in 50 nM intracellular Ca2+ concentration. La3+ (100 μM) was added at the indicated times. I-V curves were taken before (black) and 5 min after (red) the addition of WNT9B in the bath solution of untransfected (j, n=6 cells pooled from 2 independent experiments), PKD1/TRPP2- (k, n=12 cells pooled from 4 independent experiments), or PKD1S99I/TRPP2-co-transfected CHO-K1 cells (l, n=6 cells pooled from 4 independent experiments). Representative time courses (d-f) and step currents (g-i) from 6 (untransfected), 12 (PKD1+TRPP2), or 6 (PKD1S99I+TRPP2) cells. All statistical tests were performed using paired Student's t-test, **P<0.01. Data are shown as mean ± SEM. Image published in: Kim S et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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