Xenbase Image ID: 155101

Figure 3. Pathogenic mutations in PKD1 or TRPP2 suppress activation by WNT9B(a-h) I-V curves taken before (black) and 5 min after the addition of WNT9B (500 ng/ml) in the bath solution (red) of untransfected (a, n=5 cells pooled from 2 independent experiments), PKD1/TRPP2- (b, n=8 cells from 3 independent experiments), or PKD1S99I/TRPP2- (c, n=7 cells from 3 independent experiments), PKD1/TRPP2D511V- (d, n=11 from 4 independent experiments), PKD1/TRPP2R872X- (e, n=7 cells from 3 independent experiments), PKD1/TRPP2Kv1.3- (f, n=12 cells from 5 independent transfections), PKD1L1/TRPP2- (g, n=9 cells from 5 independent experiments), or PKD1/TRPP2/ZNRF3- (h, n=11 cells pooled from 3 independent transfections) co-transfected CHO-K1 cells in zero intracellular Ca2+. (i) Summary data of inward (−100 mV) or outward currents (+100 mV) from all groups. Differences between current densities before and after WNT9B for any given membrane potential in I-V curves were determined using paired Student's t-test, *P<0.05, **P<0.01. Data are shown as mean ± SEM. Image published in: Kim S et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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