Xenbase Image ID: 155102

Figure 4. TRPP2 mediates WNT9B-induced whole cell currents in MEFs(a-c) Time course (a), step currents (b), and I-V curves of WNT9B-induced whole cell currents in 50 nM intracellular Ca2+ (c, n=7 cells pooled from 3 independent experiments), before (Cont-Pkd2+/+) or after WNT9B (500 ng/ml, WNT9B-Pkd2+/+) in wild type Pkd2+/+ MEFs. Time course of WNT9B-induced currents in Pkd2−/− MEFs is shown as open blue circles in (a), whereas I-V curves are shown as open black (Cont-Pkd2−/−) and open blue circles (WNT9B-Pkd2−/−) in (c) (n=6 cells from 3 independent experiments). (d-l) Time course, step currents, and I-V curves of WNT9B-induced whole cell currents in zero intracellular Ca2+ in mock- transfected (just CD8α) (d-f, n=6 cells from 3 independent experiments), wild type TRPP2- transfected (g-i, n=12 cells from 5 experiments), or TRPP2Kv1.3- transfected Pkd2−/− cells (j-l, n=5 cells pooled from 3 independent experiments). Time course and I-V curves of WNT9B-induced currents in zero intracellular Ca2+ in Pkd2−/− cells is shown as open blue circles in (d) and (f, n=6 cells pooled from 3 independent experiments). (m-o) Time course, step currents, and I-V curves of WNT9B-induced whole cell currents in zero intracellular Ca2+ in ZNRF3-transfected Pkd2+/+ MEFs (n=12 cells pooled from 4 independent experiments). Step currents are shown 5 min after the addition of WNT9B (500 ng/ml) in the bath solution. I-V curves were taken before (black) and 5 min after the addition of WNT9B (red). Differences between current densities before and after WNT9B for any given membrane potential in I-V curves were determined using paired Student's t-test, *P<0.05, **P<0.01. Data are shown as mean ± SEM. Representative images of time courses (a,d,g,j,m) and step currents (b,e,h,k,n) were obtained from 7, 6, 12, 5, 12 cells, respectively. Image published in: Kim S et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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