Xenbase Image ID: 155105

Figure 7. TRPP2 mediates WNT9B-induced cell migration(a) Pkd2+/+ cells stained with phalloidin (green, to visualize F-actin), Myosin IIB (red), or DAPI (blue) at 0 (Control) or 30 min after the addition of WNT9B (500 ng/ml). Arrow heads indicate accumulation of F-actin and Myosin IIB in the trailing edge of a migrating cell. Scale bar, 50 μm. (b) Percentage of Pkd2+/+ cells with trailing edge per field (total number of fields of cells pooled from 3 independent experiments shown in graph) incubated with WNT9B or PBS at different time points. (c) Percentage of cells with trailing edge per field (total number of fields of cells pooled from 3 independent experiments shown in graph) in Pkd2+/+ MEFs incubated with WNT9B in the presence or absence (DMSO) of 10 μM BAPTA/AM at different time points. (d) Representative images of GFP-, GFP+TRPP2WT- or GFP+TRPP2Kv1.3-transfected Pkd2−/− cells stained for F-actin (yellow) and Myosin IIB (red). Scale bar, 50 μm. (e) Percent of migrating GFP-positive cells in three groups treated for 1 h with PBS or WNT9B (500 ng/ml) from one representative out of three independent transfections (total number of fields of cells from a single experiment, that was independently repeated three times are shown in graph). Statistics source data for all three experiments are available in Suppl. Table 3. (f) Cells migrated through a trans-well filter in the presence of WNT9B (1 μg/ml) or PBS for 6 h. Scale bar, 500 μm. (g) Number of migrating Pkd2+/+ or Pkd2−/− cells through a trans-well filter in the presence of PBS or WNT9B. n= 18 fields per group pooled from 3 independent experiments scoring a total of 2462 (Pkd2+/+-PBS), 5665 (Pkd2+/+-WNT9B), 3091 (Pkd2−/−-PBS), or 2796 (Pkd2−/−-WNT9B) cells. (h) Number of migrating cells in the presence of PBS or PDGF-BB (25 ng/ml). Data were pooled from 3 independent experiments in quadruplicates (n= 12 fields per group scoring a total of 3625 (Pkd2+/+-PBS), 8927 (Pkd2+/+-PDGF-BB), 3904 (Pkd2−/−-PBS), or 9048 (Pkd2−/−-PDGF-BB) cells. N.S means non-significant, *P<0.05, ***P<0.001 in one-way ANOVA followed by Neuman-Keuls post hoc test. Data are shown as mean ± SEM. Image published in: Kim S et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

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