Xenbase Image ID: 155106

Figure 8. Cooperativity between PKD1 and DVL2 in Xenopus(a-l) Xenopus embryos were injected with the indicated amounts of Pkd1-sMO1, Dvl2-MO and Wnt9A-MO either alone or in combination. Embryos were analyzed at stage 43 by morphology for edema formation or at stage 40 by immunofluorescence with the proximal tubular marker 3G8 (green) and the distal tubule/nephric duct marker 4A6 (yellow). Representative images of an uninjected control (a,e) and embryos injected with suboptimal amounts of Pkd1-sMO1 (b,f), Dvl2-MO (c,g), a mixture of Pkd1-sMO1 and Dvl2-MO (d,h), as well as with a Wnt9A-sMO alone (j) or in combination with suboptimal amounts of Pkd1-sMO1 (k) are shown. Bar diagrams showing the percentage of embryos exhibiting edema or dysplastic kidneys from 4 or 3 independent experiments are shown in (i) or (l), respectively. (m) Rescue experiment of Dvl2 morphants with a unilateral injection of either 4 ng wild type human DVL2 mRNA or 4 ng mutant DVL2-E499G mRNA. Embryos were analyzed at stage 40 by 3G8/4A6 immunofluorescence comparing the mRNA injected side with the contralateral side. Bar diagram depicts the summary of 3 independent experiments. (Inset) Expression levels of Flag-tagged human DVL2 and DVL2-E499G mutant in stage 25 embryos injected with 1 ng mRNA into a single blastomere at the 4-8 cell stage. Experiment was done twice. Total number of scored embryos is shown on top of each bar. Image published in: Kim S et al. (2016) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View

Return to previous page