Xenbase Image ID: 155157

FIGURE 4:. MT regrowth does not impair spindle assembly in X. laevis egg extracts. (A) Schematic representation of spindle assembly in cycled Xenopus egg extract (Control) and upon MT regrowth after cold treatment (MT Regrowth). Time is in minutes. (B) Representative images of mitotic structures assembled in Xenopus egg extracts in control conditions and after cold-induced MT depolymerization (MT Regrowth) at the indicated times (in minutes) as shown in A. At 60 min, bipolar spindles assemble in both conditions. DNA is in blue and tubulin in red. Scale bar, 10 μm. (C) Control and MT regrowth extracts assemble spindles with similar efficiency. Spindle organization in control egg extracts (blue) or cold-treated egg extracts (MT Regrowth, red). Proportion of spindle organizations after 60 min of incubation. Representative images are shown below the graph. Data from 321 control structures and 312 MT regrowth structures from three independent experiments, counting in each at least 100 mitotic structures/condition. Error bars, SEM. DNA is in blue and tubulin in red. Scale bar, 10 μm. No statistically significant difference was detected between conditions. Image published in: Cavazza T et al. (2016) © 2016 Cavazza et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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