Xenbase Image ID: 155158

FIGURE 5:. Reduced tubulin amounts impair spindle assembly in X. laevis egg extracts undergoing MT regrowth. (A) Spindles assembled in the presence of 1.2 μM nocodazole are shorter and have reduced tubulin density. Left, representative images of spindles assemble in control extract (0 μM, blue) and nocodazole-treated extract (1.2 μM, red). Right, mean tubulin density and mean spindle length as percentage of control (0 μM). Data from 172 control and 158 nocodazole-treated spindles from three independent experiments, quantifying in each at least 50 spindles/condition. DNA is in blue and tubulin in red. Scale bar, 10 μm. **p < 0.01. (B) Representative images of mitotic structures assembled in Xenopus egg extracts treated or not with 1.2 μM nocodazole in control (Control) conditions and after cold-induced MT depolymerization (MT Regrowth) at the indicated times (in minutes), treated as shown in Figure 5A. At 60 min, bipolar spindles assemble in all conditions but with different efficiencies. DNA is in blue and tubulin in red. Scale bar, 10 μm. (C) MT regrowth compromises spindle assembly only in extracts treated with nocodazole. Spindle organization in control egg extracts (blue) or cold-treated egg extracts (MT Regrowth red), treated (diagonal lines pattern) or not (no pattern) with 1.2 μM nocodazole as indicated. Proportion of spindle organizations after 60 min of incubation. Representative images are shown below the graph. Data from 479 control structures, 648 MT regrowth structures, 706 control in the presence of nocodazole, and 778 MT regrowth in the presence of nocodazole structures from four independent experiments, counting in each at least 100 mitotic structures/condition. Error bars: SEM. DNA is in blue and tubulin in red. Scale bar, 10 μm. *p < 0.05 and **p < 0.01. Image published in: Cavazza T et al. (2016) © 2016 Cavazza et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license Larger Image Printer Friendly View

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