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Figure 2. Cp110 is required for primary and motile monocilia.(A) Cp110 is required for Hedgehog signaling-dependent nkx2.2 expression. Whole-mount in situ hybridization (WMISH) staining for nkx2.2 expression in the neural tube. Normal expression indicated by green arrowhead, reduced expression indicated by red arrowhead. Related to Figure 2—figure supplement 1A–C (white and black boxes indicate normal and reduced expression, respectively in graph Figure 2—figure supplement 1A). (B) Cp110 is required for GRP cilia. Immunofluorescent staining for cilia (Ac.-α-tub., white) and cell borders (Actin, red). Green arrowheads, normal cilia; red arrowheads, defective cilia. Related to Figure 2—figure supplement 1D–F. (C) cp110MO interferes with left side specific pitx2c expression in the lateral plate mesoderm as shown by WMISH. Green arrowhead, normal/left expression; red arrowhead, absent expression. Related to Figure 2—figure supplement 1G. See also:DOI: http://dx.doi.org/10.7554/eLife.17557.008Figure 2—figure supplement 1. Cp110 is required for primary and motile monocilia.(A–C) Related to Figure 2A. Control and cp110 morphant embryos were stained by WMISH and cleared embryos were analyzed for nkx2.2 and pax6 expression in the neural tube. (A) Quantification of nkx2.2 expression. (B) WMISH for pax6 expression. Normal expression indicated by green arrowhead, reduced expression indicated by red arrowhead. White and black boxes indicate normal and reduced expression, respectively in graph in C. (C) Quantification of pax6 expression. In (A) and (C), ***p<0.001 from χ²-test. (D–F) Related to Figure 2B. Quantification of GRP ciliation rate (D), GRP cilia length (E), and GRP cilia polarization (F) in central GRP regions from one representative experiment. In (D) and (E), ***p<0.001; **p<0.01; from Wilcoxon two-sample test. In (F), ***p<0.001 from χ²-test. (G) Related to Figure 2 C. Quantification of pitx2c expression. In G, ***p<0.001 from χ²-test.DOI: http://dx.doi.org/10.7554/eLife.17557.009 Image published in: Walentek P et al. (2016) © 2016, Walentek et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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