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Figure 3—figure supplement 3. Cep97 does not localize to cilia-forming basal bodies.(A–C) GFP-Cep97 localizes centrioles of epidermal cells in Xenopus, but does not localize to basal bodies in MCCs. (A) GFP-Cep97 (green) localizes to centrioles (Centrin4-CFP, blue; Clamp-RFP, red) of epidermal cells (inset). n = 3 embryos. (B) GFP-Cep97 (green) does not localize to basal bodies/rootlets in MCCs (Centrin4-CFP, blue; Clamp-RFP, red). n embryos/MCCs: 3/9. (C) Lateral projection of MCC shown in (B) shows primarily cytoplasmic localization of GFP-Cep97. (D) Overexpression of gfp-cep97 does not inhibit cilia in Xenopus MCCs. n embryos/MCCs: 3/15.DOI: http://dx.doi.org/10.7554/eLife.17557.013

Image published in: Walentek P et al. (2016)

© 2016, Walentek et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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