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Figure 4—figure supplement 2. Cp110 is required for ciliary adhesion complex formation in MCCs.(A-D) Cp110 is required for Vinculin (n embryos/MCCs: control (10/30), cp110MO (10/30)) and Paxillin (n embryos/MCCs: control (5/15), cp110MO (5/15)) localization to MCC basal bodies. Control and cp110 morphant embryos were injected with vinculin-gfp (A) and (C), green) or paxillin-gfp (B) and (D), green) together with centrin4-cfp (blue). Related to Figure 4C–D. (E) Full membranes of co-IP experiment shown in Figure 4B. In addition to FLAG-Cp110 (full length Cp110), a FLAG-Cp110-FSΔCCD1 was overexpressed, which misses the first coiled-coil domain, as well as the truncation of the cp110-fs clone (please compare to Figure 5D).DOI: http://dx.doi.org/10.7554/eLife.17557.017 Image published in: Walentek P et al. (2016) © 2016, Walentek et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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