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Fig. 3. Immunocytochemistry of X. tropicalis testicular cell culture expressing Katushka RFP. Positive staining for Sma (marker of peritubular myoid cells, green) (A) and vimentin (marker of mesenchymal cells and peritubular myoid cells, green) (B). Nuclei were counterstained with DAPI (blue). (C-E) Double staining with Sox9 (marker of Sertoli cells, green) (C) and Sma (blue) (D) antibodies. (E) Merge of C and D. Yellow arrowhead, cell expressing both antigens; red arrowheads, cells expressing only Sox9 or only Sma. (F-H) Double staining with Sox9 (green) (F) and vimentin (blue) (G) antibodies. (H) Merge of F and G. Scale bars: 50 μm. Image published in: Tlapakova T et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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