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Fig. 4. Immunohistochemistry of agarose embedded testicular sections from X. tropicalis and mouse adult males. (A,C) Double staining with Sox9 (red) and Sma (green) antibodies. White arrowheads indicate potential common precursor cells for Sertoli and PTM cell lineages expressing both antigens in X. tropicalis (A) and mouse (C) samples. Insets show a detailed view of structures marked with white arrowheads in underlying figures. (B,D) Staining with vimentin (green) antibody on X. tropicalis (B) and mouse (D) samples. Nuclei were counterstained with DAPI (blue). Scale bars: 40 μm. Image published in: Tlapakova T et al. (2016) © 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue acta2.L a-sma, aat6, actsa, alpha smooth muscle actin, alpha-actin, alpha-SM-actin, ASMA, mymy5, SM actin, sm-actin, sma, SMactin X. laevis Throughout adult frog stage testis sox9.L cmd1, cmpd1, sox-9, sox9-a, sox9-b, sra1 X. laevis Throughout adult frog stage testis Image source: Published Larger Image Printer Friendly View

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