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Figure 3—figure supplement 2. ECD8 does not direct GFP-Pk3 polarization.Eight-cell embryos were injected at the left ventral-animal blastomere with RNAs encoding GFP-Pk3 and HA-Vangl2, followed by injection of RNAs for TFP and either Wnt5a (500 pg) or ECD8 (500 pg) into the right ventral-animal blastomere. GFP and TFP fluorescence in the epidermal ectoderm of stage 11.5 embryos is shown. Images are representative of two independent experiments. Scale bar, 20 µm.DOI: http://dx.doi.org/10.7554/eLife.16463.008 Image published in: Chu CW and Sokol SY (2016) © 2016, Chu et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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