Xenbase Image ID: 155268

Figure 4. Analyzing mutations in F0 generation tadpoles produced with a TALEN targeting Sox3 gene in Xenopus tropicalis by using pmCherry-GFP construct.(A) Diagram showing the strategy to clone a genomic DNA fragment encompassing the region targeted by TALEN in the Xenopus tropicalis Sox3 gene into pmCherry-GFP construct. (B) Three different tadpoles generated from eggs injected with Sox3 TALEN have different rate of mutations in the Sox3 gene. The mRNAs encoding the left and right arms of a TALEN targeting the Sox3 region shown in (A) were injected into fertilized egg and the animals were reared into tadpole stage. The genomic DNA was isolated from one wild type (Wt) and 3 different F0 Sox3 TALEN tadpoles (M1-3), cloned into pmCherry-GFP construct and analyzed as shown in Figs 2 and 3. Note that all colonies from the Wt sample were yellow in the merged image while for the mutant samples, some were red and some were yellow. Image published in: Fu L et al. (2016) Copyright © 2016, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page